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The I.M.A.G.E. Consortium has arrayed cDNA clones for various EST projects. A majority of the clones arrayed by I.M.A.G.E. Consortium are available royalty-free through our distributors (see the FAQ for more information), and all sequences obtained are submitted immediately to dbEST. The I.M.A.G.E. Consortium team at Lawrence Livermore National Laboratory is supported by the U.S. Department of Energy (Office of Biological and Environmental Research) and the National Institutes of Health.
Merck/WashU EST Project (1995-1997)
Human clones arrayed between 1995 and 1997 were sequenced by Washington University under the sponsorship of Merck. 584,000 ESTs from 5' and 3' reads were submitted to dbEST from this project.
HHMI/WashU Mouse EST Project (1996-1998)
Mouse clones arrayed between 1996 and 1998 were sequenced by Washington University under the sponsorship of the Howard Hughes Medical Institute. Nearly 400,000 ESTs, mostly 5' reads, were submitted to dbEST from this project.
Cancer Genome Anatomy Project (1997-2004)
Human clones arrayed from 1997 on, mostly from NCI-CGAP libraries, were sequenced by Washington University and the NIH Intramural Sequencing Center (NISC), under the sponsorship of the National Cancer Institute. Beginning in 1999, the CGAP project has also contributed libraries from other species, including mouse, rat, Xenopus, and primate. SAGE (Serial Analysis of Gene Expression) libraries have also been included under the CGAP project. Sequencing of SAGE clones is done by Washington University and NISC, and analysis is done by the National Center for Biotechnology Information (NCBI).
WashU Zebrafish EST Project (1997-2002)
cDNA libraries gathered from the zebrafish community have been arrayed by I.M.A.G.E. Consortium and other sources, and sequenced at Washington University.
University of Iowa Rat EST Project (1998-2001)
Clones from this project are arrayed and sequenced at the University of Iowa, sponsored by the National Institutes of Health. Unique clones are rearrayed and given I.M.A.G.E. Consortium cloneIDs (located in the comment field of the Genbank entry). 25,000 of these sequence-verified clones are currently available through the I.M.A.G.E. Consortium distributors.
WashU Xenopus EST Project (1999-2002)
cDNA libraries gathered from the Xenopus community as well as constructed by CGAP have been arrayed by I.M.A.G.E. Consortium and other sources, and sequenced at Washington University.
NIH Mammalian Gene Collection (1999-ongoing)
The focus of the MGC project, sponsored by the National Institutes of Health, is to generate full-length cDNA resources for human and other mammalian species. Initially, clones from MGC libraries are sequenced from the 5' and 3' ends, and evaluated for full-length characteristics. Prospective full-length clones are rearrayed, verified, and then fully sequenced.
NIH Xenopus Gene Collection (2002-ongoing)
The Xenopus Gene Collection project, sponsored by the National Institutes of Health, seeks to identify and fully-sequence open reading frame clones for a representative set of Xenopus genes.
NIH Zebrafish Gene Collection (2002-ongoing)
The Zebrafish Gene Collection is an initiative sponsored by the National Institutes of Health to support the production of zebrafish cDNA libraries, clones and sequences to provide a complete set of full-coding clones.
Bovine Genome Sequencing Program (2004-ongoing)
The full length cDNA sequencing portion of this project aims to sequence 10K full coding bovine cDNA clones. The project is led by British Columbia Genome Sequencing Center and the University of Alberta, and is part of the larger Bovine Genome Sequencing Project, an international initiative to sequencing the bovine genome.
ORFeome Collaboration (2005-ongoing)
The ORFeome Collaboration (OC) was formed, in late 2005, to meet the need of the research community for an unrestricted source of fully sequence-validated full-ORF human cDNA clones in a format allowing easy transfer of the ORF sequences into virtually any type of expression vector. A major goal is to provide at least one fully sequenced full-ORF clone for each of the ~18,500 currently defined human genes. These clones are to be made available, without restriction, to researchers worldwide through multiple commercial distributors.
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